Novel Molecular Assay and Sample Preparation Method for the Detection of Alicyclobacillus in Fruit Juice Concentrates and Bottling Process Materials
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چکیده
Figure 1: GeneDisc Plate for TAB Spoilage Alicyclobacilli are thermophilic, acidophilic and spore-forming bacteria (TAB) capable of surviving extreme conditions during the fruit juice manufacturing and bottling processes. These spoilage organisms can generate strong off-flavors and off-odors, which negatively affect product quality in fruit juices, concentrates and ingredients used in beverage production. Though non-pathogenic, they pose a major economic risk to processors. Rapid detection and identification of TAB spoilage at various identified critical control points of the production process is a valuable tool to facilitate release of high quality products.
منابع مشابه
Detection of Alicyclobacillus spp. in Fruit Juice by Combination of Immunomagnetic Separation and a SYBR Green I Real-Time PCR Assay
An approach based on immunomagnetic separation (IMS) and SYBR Green I real-time PCR (real-time PCR) with species-specific primers and melting curve analysis was proposed as a rapid and effective method for detecting Alicyclobacillus spp. in fruit juices. Specific primers targeting the 16S rDNA sequences of Alicyclobacillus spp. were designed and then confirmed by the amplification of DNA extrac...
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